Figure 2.

Interaction of RARα with the first three N-terminal zinc fingers of the DNA-binding domain of PLZF. A) Bacterially expressed GST-PLZF 3ZF fusion protein was used to generate an affinity matrix with which (panel B) ³⁵S-labeled full length RARα, RARα 2 K, isolated functional domains (RARα AF1, RARα AF2) or RARα deleted of its AF2-AD and the domain F (RARα Δ403) were incubated in the absence or presence of 1 μM atRA. Receptors bound to PLZF 3ZF resin were then resolved by SDS-polyacrylamide gel electrophoresis and quantified by autoradiography using the ImageQuant software (Molecular Dynamics, Inc.).