Figure 5.

PLZF interacts physically with nuclear receptors. RARα, ERα and GR were synthesized and labeled by in-vitro coupled transcription/translation and then incubated with a GST-PLZF 3ZF-Sepharose matrix in the absence or presence of ligand, which were 1 μM atRA, 1 μM E2 and 0.1 μM Dex as indicated.