Figure 5.

PPARδ is necessary for PMA stimulated adherence of THP-1 cells. Wild-type THP-1 cells (squares), SENSE (inverted triangles) and ANTISENSE cells (circles) were plated at a density of 5 × 10⁵ cells/ml. After 48 h the cells were treated with PMA and this was maintained for a further 48 h. After this time the PMA was removed. At all stages, both adherent (A) and non-adherent cells (B) were counted with SYBR green and cell number calculated from a standard curve.