Constitutive and ligand-dependent coactivator interactions of CAR isoforms Mammalian two hybrid assays were performed in COS1 cells transiently transfected with combinations of expression plasmids encoding VP16-AD/CAR-LBD fusion proteins and GAL4-DBD/coactivator-RID fusion proteins, as indicated, together with the reporter gene plasmid pGL3-G5. The columns show the mean activation factors (± S.D.) of the GAL4-dependent reporter through interaction of GAL4-DBD/coactivator-RID fusion proteins (SRC-1, TIF-2, ACTR, DRIP205) with VP16-AD/CAR-LBD fusion proteins of the CAR isoforms SV1 to SV6. Open and filled columns indicate treatment with vehicle Me2SO and CITCO (1 μM), respectively. The activity of each GAL4-DBD/coactivator-RID fusion in the presence of the empty expression vector pVP16-AD (VP16) treated with Me2SO was designated as 1.
Arnold et al. Nuclear Receptor 2004 2:1 doi:10.1186/1478-1336-2-1