Figure 2.

ERβ structurally disrupted in the DBD re-localizes to the cytoplasm and induces AP-1 activity in the presence of 17β-estradiol. (A) HC11 cells were transiently co-transfected with 500 ng coll-73-luc reporter gene or 1 μg β-casein reporter gene and 200 ng of ERβ wt or C201A/C204A. Cells were treated with either no hormone (NH) or 10^-8 M 17β-estradiol (E2), as indicated, and the reporter activity was analysed 24 hours after treatment. Luciferase activity was normalised using β-gal as an internal control. Data are representative of at least three independent experiments performed in duplicate. Mean and ± SD are shown. (B) HC11 cells were transfected with expression vectors for ERβ wt or C201A/C204A. Cells were treated with either no hormone (NH) or 10^-8 M 17β-estradiol (E2), as indicated, and the cellular localization of the receptor proteins was analysed 24 hours after treatment by indirect immunofluorescence as described in Experimental Procedures.