Figure 3.

Ligand-dependent ER actions at an AP-1 response element. HC11 cells were transiently co-transfected with 500 ng coll-73-luc reporter gene and (A) 200 ng of NLSA or (B) ERβ 148-530 Flag. Cells were treated with either no hormone (NH), 10^-8 M 17β-estradiol (E2), 10^-7 M DES, 10^-7 M PPT, 10^-7 M estren, 10^-7 M tamoxifen (OHT), 10^-7 M raloxifene (Ral), 10^-7 M idoxifene (Idox), 10^-7 M nafoxidene (Nafox) or 10^-7 M ICI 182,780 (ICI), as indicated, and the reporter activity was analysed 24 hours after treatment. Luciferase activity was normalised using β-gal as an internal control. Data are representative of at least three independent experiments performed in duplicate. Mean and ± SD are shown. (C) HC11 cells were transiently co-transfected with 500 ng TRE-tk-luc reporter gene and 200 ng of NLSA or ERβ 148-530 Flag. Cells were treated with either no hormone (NH), 10^-8 M 17β-estradiol (E2), 10^-7 M tamoxifen (OHT) or 10^-7 M ICI 182,780 (ICI), as indicated, and further assayed as described in A and B. (D) HC11 cells were transiently co-transfected with 500 ng coll-73-luc reporter and 200 ng of ERα wt or ERβ wt. Cells were treated and analysed as described in A and B.