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Open Access Research

Diurnal difference in CAR mRNA expression

Yuichiro Kanno, Satoshi Otsuka, Takuya Hiromasa, Takayuki Nakahama and Yoshio Inouye*

Author Affiliations

Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan

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Nuclear Receptor 2004, 2:6  doi:10.1186/1478-1336-2-6

Published: 28 August 2004

Abstract

Background

The constitutive androstane receptor (CAR, NR1I3) plays a key role in the transcriptional activation of genes that encode xenobiotic/steroid and drug metabolizing enzymes.

Results

The expression of CAR mRNA throughout the circadian rhythm is reported for the first time in phase with the clock gene Bmal1 and in antiphase with the clock-controlled gene Rev-erbĪ± mRNAs, with a peak at Zeitgeber time (ZT) 20 and a trough at ZT8, and a peak/trough ratio of 2.0. The diurnal difference in CAR mRNA expression might underlie the 1.7-fold difference in the magnitude of the PB-dependent induction of CYP2B1/2 mRNA.

Conclusion

The circadian oscillation of xenosensor gene CAR mRNA expression is partially responsible for chronopharmacokinetics and chronopharmacology in disease.