Figure 2.

Lack of proteolytic activity of uremic plasma on TRβ1. Recombinant [³⁵S]TRβ1 was produced by translation in reticulocyte lysate and then incubated for 30 min at 4°C without (Control – lane 1) or with increasing volumes (0.5, 1.0, 2.0 μL) of normal (lanes 2–4) or uremic (lanes 5–7) plasma. Products were analyzed by SDS-PAGE and autoradiography.