Time course of PPAR expression in established cell lines. A, B, C and D, The cell lines for the expression of PPARα (A), PPARδ (B), PPARγ1 (C) or PPARγ2 (D) were cultured in the presence of Dox. At time point 0, Dox was removed from the medium. The total RNAs were extracted from the cells cultured for the indicated days after removal of Dox and the amount of mRNAs of PPARs were measured by real time RT-PCR. Values are expressed as the mean ± S.E.M. (n = 3), relative to the control (0 day) set as 1. E, F, G and H, Nuclear extracts (50 μg protein/lane) from the cell lines expressing PPARα (E), PPARδ (F), PPARγ1 (G) or PPARγ2 (H) cultured for the indicated days after removal of Dox were subjected to SDS-PAGE and immunoblots were performed with anti-PPARα (E), anti-PPARδ (F) or anti-PPARγ (G and H).
Tachibana et al. Nuclear Receptor 2005 3:3 doi:10.1186/1478-1336-3-3