Additional File 1.

Changes in mRNA expression levels in HepG2-tet-off-hPPAR cell lines by ligands. Microarray analyses were performed on HepG2-tet-off-hPPAR cells; the cells were cultured in the presence (Dox) or absence of Dox for 5 days. In the absence of Dox, the cells were treated with PPAR ligands (100 μM fenofibric acid for PPARα (Feno), 100 nM GW501516 for PPARδ (GW) or 10 μM ciglitizone for PPARγ (Cig)) or vehicle (DMSO) for 24 h. Gene expression profiles were compared between DMSO and Dox (DMSO versus Dox), ligands and Dox (Feno versus Dox, GW versus Dox, and Cig versus Dox were indicated in the case of PPARα, PPARδ and PPARγ, respectively) or ligands and DMSO (Feno versus DMSO, GW versus DMSO, and Cig versus DMSO were indicated in the case of PPARα, PPARδ and PPARγ, respectively). Average differences expressed the intensities of the mRNA levels in HepG2-tet-off-hPPARs cell lines. Samples were analysed using GeneChip^® software Microarray Suite (MAS) Ver.5.0 (Affymetrix).

Format: XLS Size: 92KB Download file

This file can be viewed with: Microsoft Excel Viewer

Tachibana et al. Nuclear Receptor 2005 3:3   doi:10.1186/1478-1336-3-3